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• 15:33, 30 May 2025 ESO wikiadmin talk contribs created page Cloning of guideRNA-Oligo into pLentiCRISPRv2 (Created page with "{{Protokoll-Mix|titel=Materials|Inhalt=gRNA Oligos (sense/antisense; designed using ChopChop)<br>Vector pBluescript_U6-MCS digested with BbsI (Plasmid #3468)<br>T4 DNA ligase; 10x Ligase buffer<br>E. coli NovaBlue}}")