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• 12:13, 30 May 2025 ESO wikiadmin talk contribs created page Cloning of shRNA into lentiviral vector pLKO.1 (Created page with "== Procedure == === Primer annealing === * annealing reaction contains: 1 µl sense primer (stock solution 100 µM), 1 µl antisense primer (stock solution 100 µM), 5 µl NEB buffer 2 and 43 µl ddH₂O * Annealing reaction occurs in thermocycler with the corresponding program:") Tag: Visual edit