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• 11:28, 30 May 2025 ESO wikiadmin talk contribs created page Designing PCR-Primers for LIC Cloning (Ligation independent cloning) (Created page with " == Procedure == Delineate the coding sequence (full-length or partial sequence), which you want to amplify. Sense and antisense primers should have around 18 to 22 complementary bases, depending on the AT-content (there should be 9-10 Gs or Cs in the primer- cDNA complementary region). Both primers should ideally end with a 3’-GC clamp (two G or C bases). In the antisense primer, add a STOP codon, if you want to create a protein with an N- terminal tag; omit the STOP...") Tag: Visual edit