Cloning of guideRNA-Oligo into pLentiCRISPRv2

From Hauck LabWiki

Revision as of 15:33, 30 May 2025 by ESO wikiadmin (talk | contribs) (Created page with "{{Protokoll-Mix|titel=Materials|Inhalt=gRNA Oligos (sense/antisense; designed using ChopChop)<br>Vector pBluescript_U6-MCS digested with BbsI (Plasmid #3468)<br>T4 DNA ligase; 10x Ligase buffer<br>E. coli NovaBlue}}")

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Jump to navigation Jump to search

Materials

gRNA Oligos (sense/antisense; designed using ChopChop)
Vector pBluescript_U6-MCS digested with BbsI (Plasmid #3468)
T4 DNA ligase; 10x Ligase buffer
E. coli NovaBlue

Retrieved from "https://wiki.esonto.de/index.php?title=Cloning_of_guideRNA-Oligo_into_pLentiCRISPRv2&oldid=267"