S1 - Cell culture guidelines

Getting started:

Turn on hood, open bench ⇒ needs several minutes to establish flow, wait for green light
Tie back hair, push back lose sleeves; disinfect hands & arms, put on gloves (can be reused)
Wipe down bench and window with 70% EtOH
Gather all material you need, spray with 70% EtOH and wipe down before placing under hood
Arrange items under hood towards the back of the bench → Keep in mind that you should work a minimum of 15cm inward from the outside edge of the laminar airflow hood; also, leave bottles closed until use and don’t move over open containers; no pouring, use pipettes!
Always open sterile plastic consumables under the hood

Minimize waste whenever possible, plan experiments accordingly
Clean spills immediately with 70% EtOH
Let appropriate person know if things are running low: e.g. Jan for plastic consumables, Marleen for PFA, Claudia/Susi for Trypsin, FCS/CS etc., Steffi for general S1 issues...
Thaw new trypsin bottle for next person if current bottle is close to empty
Fill up glass pasteur pipettes if you emptied a canister ⇒ stick on “heat-dry” tape & put on cart
Empty trash can if ¾ full, lightly close with tape & place on cart for autoclaving ⇒ use 2 new autoclave bags!
Collect polystyrene waste (f.ex. pipets w/o filter, cell culture dishes and multiwell plates) in SEPARATE trash can
Make sure clip on vacuum hose is on completely –> or else pump keeps running!
Plug in pipette boy if battery is low
Close bench when you step away for an extended period („stand-by mode“)
Vent vacuum when you are done (but it‘s not the end of the day yet)
Turn off computer AND monitor when done using, turn of microscope light when done using
If fluorescence lamp was used, set timer for 30min ⇒ Should not be switched back on again within 30min!
Turn on UV light under bench for 30min if a contamination is suspected! (set timer for 30min)

!!! Leave it how you would like to find it !!!