Concentration of lentiviral particles via ultracentrifugation: Difference between revisions

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Created page with "{| class="wikitable" |+ !Materials ! ! ! |- | |Virus containing supernatant | | |- | |20% sucrose in PBS | | |- | |1% BSA in PBS | | |} === Procedure === ---- # 4 ml of 20% sucrose solution are added to an ultracentrifuge tube and overlayed with 26 ml of filtrated virus containing supernatant # the tubes are placed in aerosol tight swing buckets # centrifugation is carried at 20.000 rpm , 4°C for 2h15min (Rotor: SW32Ti) # after discarding the supernatant the pellet is..."
 
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Latest revision as of 15:47, 30 May 2025

Materials
Virus containing supernatant
20% sucrose in PBS
1% BSA in PBS

Procedure


  1. 4 ml of 20% sucrose solution are added to an ultracentrifuge tube and overlayed with 26 ml of filtrated virus containing supernatant
  2. the tubes are placed in aerosol tight swing buckets
  3. centrifugation is carried at 20.000 rpm , 4°C for 2h15min (Rotor: SW32Ti)
  4. after discarding the supernatant the pellet is resuspended in 250µl 1%BSA
  5. Aliquot and freeze in liquid nitrogen
  6. Store at -80°C (repeated freezing and thawing might result in titer reduction)