S1 - Cell culture guidelines: Difference between revisions
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Created page with "'''Getting started:''' * Turn on hood, open bench needs several minutes, wait for green light * Tie back hair, push back lose sleeves; disinfect hands & arms, put on gloves (can be reused) * Wipe down bench and window with 70% EtOH * Gather all material you need, spray with 70% EtOH and wipe down before placing under hood * Arrange items under hood towards the back of the bench ⇒Keep in mind that you should work a minimum of 15cm inward from the outside edge of th..." |
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* Arrange items under hood towards the back of the bench ⇒Keep in mind that you should work a minimum of 15cm inward from the outside edge of the laminar airflow hood; also, leave bottles closed until use and don’t move over open containers; no pouring, use pipettes! | * Arrange items under hood towards the back of the bench ⇒Keep in mind that you should work a minimum of 15cm inward from the outside edge of the laminar airflow hood; also, leave bottles closed until use and don’t move over open containers; no pouring, use pipettes! | ||
* Always open sterile plastic consumables under the hood | * Always open sterile plastic consumables under the hood | ||
[[File:Cellculture meme.jpg|thumb]] | |||
'''Before you sit down''' '''& when you are done''': If necessary | '''Before you sit down''' '''& when you are done''': If necessary | ||
* Stock up pipettes and falcons (also glass pasteur pipettes) | * Stock up pipettes and falcons (also glass pasteur pipettes) | ||
* Refill EtOH bottles (also canister, if empty) | * Refill EtOH bottles (also canister, if empty) | ||
Revision as of 16:24, 3 June 2025
Getting started:
- Turn on hood, open bench needs several minutes, wait for green light
- Tie back hair, push back lose sleeves; disinfect hands & arms, put on gloves (can be reused)
- Wipe down bench and window with 70% EtOH
- Gather all material you need, spray with 70% EtOH and wipe down before placing under hood
- Arrange items under hood towards the back of the bench ⇒Keep in mind that you should work a minimum of 15cm inward from the outside edge of the laminar airflow hood; also, leave bottles closed until use and don’t move over open containers; no pouring, use pipettes!
- Always open sterile plastic consumables under the hood
Before you sit down & when you are done: If necessary
- Stock up pipettes and falcons (also glass pasteur pipettes)
- Refill EtOH bottles (also canister, if empty)
- Check vacuum bottles
- Turn vacuum on or off, if applicable
General lab maintenance/ behaviour:
- Clean spills immediately
- Minimize waste: Think ahead and plan experiment accordingly
- Plug in pipette boy if battery is low
- Thaw new trypsin bottle if close to end
- Let appropriate person know if things are running low: e.g. Jan for plastic consumables, Marleen for PFA, Claudia/Susi for Trypsin, serum etc., Steffi for general S1 issues...
- Fill up glass pasteur pipettes if you emptied a canister
- Collect polystyrene lab waste (f.ex. pipets w/o filter, cell culture dishes and multiwell plates) in separate trash can
- Empty trash can if full, place on cart for autoclaving
- Make sure clip-on vacuum hose is on completely –> or else pump keeps running!
- Close bench when you step away for an extended period („stand-by mode“)
- Vent vacuum when you are done (but it‘s not the end of the day yet)
- Turn off computer AND monitor when done using, turn of microscope light when done using
- If fluorescence lamp was used, set timer for 30min Should not be switched back on again within 30min!
- Turn on UV light under bench for 30min if a contamination is suspected! (set timer for 30min)