Concentration of lentiviral particles via ultracentrifugation: Difference between revisions
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Created page with "{| class="wikitable" |+ !Materials ! ! ! |- | |Virus containing supernatant | | |- | |20% sucrose in PBS | | |- | |1% BSA in PBS | | |} === Procedure === ---- # 4 ml of 20% sucrose solution are added to an ultracentrifuge tube and overlayed with 26 ml of filtrated virus containing supernatant # the tubes are placed in aerosol tight swing buckets # centrifugation is carried at 20.000 rpm , 4°C for 2h15min (Rotor: SW32Ti) # after discarding the supernatant the pellet is..." |
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Latest revision as of 15:47, 30 May 2025
| Materials | |
|---|---|
| Virus containing supernatant | |
| 20% sucrose in PBS | |
| 1% BSA in PBS |
Procedure
- 4 ml of 20% sucrose solution are added to an ultracentrifuge tube and overlayed with 26 ml of filtrated virus containing supernatant
- the tubes are placed in aerosol tight swing buckets
- centrifugation is carried at 20.000 rpm , 4°C for 2h15min (Rotor: SW32Ti)
- after discarding the supernatant the pellet is resuspended in 250µl 1%BSA
- Aliquot and freeze in liquid nitrogen
- Store at -80°C (repeated freezing and thawing might result in titer reduction)