Whole cell lysates (WCLs) of eukaryotic cells: Revision history

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27 February 2025

  • curprev 14:1014:10, 27 February 2025 ESO wikiadmin talk contribs 916 bytes +916 Created page with "{| class="wikitable" |+ !Materials ! |- | |PBS 1X |- | |RIPA buffer |- | |Sepharose Beads |} === Procedure === ----All following steps are performed in the cold room: * Aspirate cell culture medium with a pasteur pipette * Rince attaching cells carefully with cold PBS * Add 1 mL RIPA buffer per 10 cm dish or 0.5 mL per 6 cm dish and incubate for approx. 2 min at 4°C * Carefully detach the cells with a cell scraper and transfer the lysate in a fresh tube * Mechanical s..." Tag: Visual edit
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