Western Blot: wet blot: Revision history

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27 February 2025

  • curprev 14:2414:24, 27 February 2025 ESO wikiadmin talk contribs 1,823 bytes 0 No edit summary Tag: Visual edit
  • curprev 14:2314:23, 27 February 2025 ESO wikiadmin talk contribs 1,823 bytes +1,823 Created page with "{| class="wikitable" |+ !Materials ! |- |Western Transfer Buffer |dissolve 6.0 g Tris Base; 28.8 g glycine in 1 L dH<sub>2</sub>O add 430 mL methanol and degas for 30 min while stirring after degasing, add 10 mL 20% SDS and fill up to 2 L with H<sub>2</sub>O |- |Blocking Solution |TBS-T; 2% BSA; 0.05% NaN<sub>3</sub> |- |TBS-Tween |200 mL 10x TBS; 10 mL Tween; ad 2 L H<sub>2</sub>O |- | |First and second Antobody |- | |ECL-Medium |- | |H<sub>2</sub>O<sub>2</sub> (30%) |-..." Tag: Visual edit
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