Western Blot: probing of the membrane: Revision history

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27 February 2025

  • curprev 14:2914:29, 27 February 2025 ESO wikiadmin talk contribs 1,855 bytes +1,855 Created page with "{| class="wikitable" |+ !Materials ! |- |Staining Solution |250 mL isopropanol; 100 mL acetic acid; 650 mL H<sub>2</sub>O; 0.03% Coomassie |- |Destaining Solution |10% (v/v) isopropanol, 10% (v/v) acetic acid |- |Blocking Solution |TBS-T; 2% BSA; 0.05% NaN<sub>3</sub> |- |TBS-Tween |200 mL 10x TBS; 10 mL Tween; ad 2 L H<sub>2</sub>O |- | |ECL-Solution |- | |H<sub>2</sub>O<sub>2</sub> (30%) |} === Procedure === ---- * Upon completed transfer of the proteins to the PVDF..." Tag: Visual edit
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