Transformation of plasmids in E. coli: Revision history

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2 June 2025

  • curprev 08:4208:42, 2 June 2025 ESO wikiadmin talk contribs 1,266 bytes +376 No edit summary Tag: Visual edit
  • curprev 08:3908:39, 2 June 2025 ESO wikiadmin talk contribs 890 bytes +890 Created page with " == Procedure == * Before transformation, appropriate aliquots of competent bacteria should be thawn on ice. * Add the plasmid DNA to 100 μl competent E. coli in a snap-cap tube and mix well. * Incubate the samples 30 min on ice. * Transfer samples for 75 seconds to a 42°C water bath or heating block and quickly back on ice. * Add 1 ml LB-Medium and incubate the bacteria for 1h at 37°C (220 rpm). * Transfer the bacterial suspension from the snap-cap tube to Eppendorf..." Tag: Visual edit
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