Transduction and titration of lentiviral supernatant: Revision history

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15 February 2025

  • curprev 18:4818:48, 15 February 2025 ESO wikiadmin talk contribs 718 bytes +718 Created page with "{| class="wikitable" |+ !Materials ! |- | |DMEM + 10% CS |- | |Puromycin (stock solution 5 mg/ml) |} === Procedure === # 1x10<sup>4</sup> 293T cells are seeded in a 24-well plate ~12 h before transduction (5 wells) # 1µl, 2µl or 5µl, respectively, of the virus-concentrate are added on top of the cells, 2 wells remain untreated # incubation over night at 37°C and 5% CO<sub>2</sub> # 24 h after transduction the medium is replaced by 1 ml fresh medium containing 10% C..." Tag: Visual edit
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