Testexpression in Pichia pastoris: Revision history

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5 June 2025

  • curprev 14:2014:20, 5 June 2025 ESO wikiadmin talk contribs 1,506 bytes +450 No edit summary Tag: Visual edit
  • curprev 14:1714:17, 5 June 2025 ESO wikiadmin talk contribs 1,056 bytes +1,056 Created page with " == Procedure == * Inoculate 5 mL YPD medium with one '''big''' colony ** in 50 ml falcon tube (secure lid with tape, don’t tighten lid) * Grow o. n. 30 °C, 200 rpm * Centrifuge at 2000 g, 5 min * Resuspend the complete pellet in 5 mL BMMY medium * Measure the OD ** Use a 1:100 dilution for measurement * Start growth ** For starters you don’t need to dilute your suspension ** For optimization purposes you may want to adjust your starting OD *** Recommended is a sta..." Tag: Visual edit
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