Site directed mutagenesis: Revision history

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30 May 2025

  • curprev 15:4115:41, 30 May 2025 ESO wikiadmin talk contribs 1,879 bytes +267 No edit summary Tag: Visual edit
  • curprev 15:4015:40, 30 May 2025 ESO wikiadmin talk contribs 1,612 bytes +1,612 Created page with "== Procedure == * Prepare the PCR reaction mix according to the hot start procedure. Include one negative control sample without Pfu polymerase (incubate sample at the lowest annealing temperature of your gradient) * Perform gradient PCR using the following thermal cycling conditions: {| class="wikitable" !Step !Temperature °C !Time !No. of cycles |- |Initial denauturation |94 |1-3 min |1 |- |Hot Start / add enzyme |94 |pause |1 |- |Denauturation |94 |20 sec | rowspan..." Tag: Visual edit
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