SEAP Reporter Assay: Revision history

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27 February 2025

  • curprev 13:4113:41, 27 February 2025 ESO wikiadmin talk contribs 1,217 bytes +1,217 Created page with "{| class="wikitable" |+ !Materials ! |- | |Eppi centrifuge at RT Heat block @65°C Substrate buffer @+4°C pNPP powder Calf Intestine Alkaline Phosphatase (CIAP) [stock: 1μL/mL, 1:1000 dilution) Multichannel pipette and plastic box for solution Spectrophotometer (VarioSkant) |- |Assay buffer (100ml) |100 mM Glycin pH 10.4 [10 M] ▶ 1 mL 1 mM ZnCl2 [1 M] ▶ 400 μL 1 mM MgCl2 [1 M] ▶ 400 μL Adjust pH with NaOH to pH 10.4 |} === Procedure === ---- * Transfer  ..." Tag: Visual edit
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