Restriction digest of plasmid DNA: Revision history

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21 May 2025

  • curprev 14:1414:14, 21 May 2025 ESO wikiadmin talk contribs 1,054 bytes +1,054 Created page with "{| class="wikitable" |+ !Materials |- |Restriction enzyme stock (–20°C) |- |Appropriate 10x enzyme buffer (–20°C) |- |Sterile dd H<sub>2</sub>O |- |Depending on the digestion '''(optional)''': '''''BSA (bovine serum albumin)''''': To ensure 100% activity of the enzymes, 100 µg/ml BSA should be added (stored at –20°C) |} == Procedure for analysis purposes == * 300 ng of DNA samples (e.g. control digest of plasmid minipreps) * 1 µl appropriate enzyme buffer (1..." Tag: Visual edit
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