Preparation of samples for confocal microscopy: Revision history

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7 March 2025

  • curprev 10:4510:45, 7 March 2025 ESO wikiadmin talk contribs 1,533 bytes +1,533 Created page with "{| class="wikitable" |+ !Materials ! |- | |Coverslips (round, 12 mm) |- | |24-well plate |- | |4% Paraformaldehyde (PFA) in PBS or |- | |ice-cold methanol or acetone (-20°C) in porcelain well plate |} === Procedure === ---- Cells: * One day before seeding the cells: distribute cleaned and sterilized coverslips in a 24-well plate and coat with gelatine-, poly-L-lysine (10 µg/mL), or fibronectin (2 µg/mL) solution in PBS overnight (o/n) at 4°C * Aspirate coating so..." Tag: Visual edit
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