Opsonizing bacteria with Fc fusion proteins: Revision history

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5 June 2025

  • curprev 13:4813:48, 5 June 2025 ESO wikiadmin talk contribs 599 bytes +599 Created page with "Bacteria are stained with CFSE and resuspended in PBS. 0.3 bacteria (OD 550) are incubated with either 0.05 mg of purified fusion proteins of 1ml of the unpurified fusion protein for 1 h under rotation at 4°C. As a positive control bacteria are incubated with 500 µl of anti-gonococcal sera (heat-inactivated for 1 h at 56°C) and 500 µl PBS. As a negative control bacteria are incubated with 1 ml of PBS. After the incubation the bacteria are washed and resuspended in 1..." Tag: Visual edit
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