Measurement of lipid raft localization via flow cytometry: Revision history

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27 February 2025

  • curprev 13:4513:45, 27 February 2025 ESO wikiadmin talk contribs 1,060 bytes +1,060 Created page with "{| class="wikitable" |+ !Materials ! |- | |PBS (pH 7.4) Trypsin/EDTA Triton-X100 Serum starvation medium: 0.5% calf serum (CS) FACS buffer: 46.5 ml PBS + 2.5 ml FCS (h.i) + 1 ml 5% sodium azide |} === Procedure === ---- * Add cells in poly-L-lysin coated dishes * Optionally: Serum starvation of cells over night, 37 °C * Positive control: fluorescently labelled choleratoxin for staining of Gm1 * Negative controls: staining of transferrin receptor, untransfected cells..." Tag: Visual edit
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