Measurement of endocytosis via reversible surface biotinylation: Revision history

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27 February 2025

  • curprev 13:5313:53, 27 February 2025 ESO wikiadmin talk contribs 1,025 bytes +33 No edit summary Tag: Visual edit
  • curprev 13:5313:53, 27 February 2025 ESO wikiadmin talk contribs 992 bytes +992 Created page with "{| class="wikitable" |+ !Materials ! |- |Biotinylation buffer: |PBS++ (pH 7.4) + 400 µg/ml Sulfo-NHS-SS- Biotin (freshly added) |- |Quenching buffer: |PBS++ (pH 7.4) + 100 mM Glycin |- |Stripping buffer: |50 mM Tris (pH 8.6), 100 mM NaCl, freshly added: 0.2 % BSA, 100 mM MESNa |- |Serum starvation medium: |DMEM + 0.5 % calf serum (CS) |} === Procedure === ---- * 2 x 105 cells in 6 cm dishes, serum starvation over night * Wash 3x with cold PBS++ at 4°C * Incubatio..." Tag: Visual edit
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