Isolation of human granulocytes: Revision history

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15 February 2025

  • curprev 18:0618:06, 15 February 2025 ESO wikiadmin talk contribs 1,992 bytes +1,992 Created page with "{| class="wikitable" !Materials ! |- | |Biocoll |- | |3.8% citrate solution PBS |- | |PVA 7200 polyvinyl alcohol 2x PBS, pH 7.4 |- | |Bidest H<sub>2</sub>O |- | |Phagocyte buffer (PBS, 0.9 mM CaCl<sub>2</sub>; 0.5 mM MgCl<sub>2</sub>; 5 mM glucose; 1% h.i FCS) |} === Preparations === PVA1% PVA 7200 + 0.9% NaCl * Add 5 g PVA to 500 ml 0.9 % NaCl solution * Solve with stirring on a heating plate * Autoclave * Cool under stirring * Store on shelf, protected from light =..." Tag: Visual edit
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