InFusion-cloning (Fa. Clontech): Revision history

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30 May 2025

  • curprev 15:4615:46, 30 May 2025 ESO wikiadmin talk contribs 711 bytes +711 Created page with "{{Protokoll-Mix|titel=Materials|Inhalt=InFusion Kit Becton-Dickinson<br>Aqua bidest<br>Linearised vector (pDNR-Dual), 100ng/1µl (~4900bp)<br>Fragment (10-50ng/µl)<br>-> Subsequent method: Transformation}} == Procedure == * fragment concentration (fragment : pDNR-Dual  2:1 ratio [fmol]) * solution of a dry-down reaction in 102 µl aqua bidest * add 14 µl 10x In-Fusion-buffer and 2 µl pDNR-Dual (200 ng) * disperse to 15 tubes (8 µl per tube) and add 0.5 -1 µl of..." Tag: Visual edit
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