Immunofluorescence staining of focl adhesion proteins: Revision history

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20 May 2025

7 March 2025

  • curprev 10:4910:49, 7 March 2025 ESO wikiadmin talk contribs 1,846 bytes +1,846 Created page with "Materials PBS++ (1x PBS + 0.9mM CaCl<sub>2</sub> + 0.5 mM MgCl<sub>2</sub>) Coverslips (round, 12 mm) 24 well plate 4% paraformaldehyde (PFA) in PBS Permeabilization/fixation solution (4% PFA/PBS + 0.1% Triton-X-100) Blocking solution (10% heat inactivated CS in PBS) Mounting medium {| class="wikitable" |+ !Materials ! |- | |PBS++ (1x PBS + 0.9mM CaCl<sub>2</sub> + 0.5 mM MgCl<sub>2</sub>) |- | |Coverslips (round, 12 mm) |- | |24 well plate |- | |4% paraformaldehyd..." Tag: Visual edit
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