Granulocyte FACS phagocytosis assay: Revision history

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15 February 2025

  • curprev 18:1118:11, 15 February 2025 ESO wikiadmin talk contribs 2,650 bytes −20 No edit summary Tag: Visual edit
  • curprev 18:1018:10, 15 February 2025 ESO wikiadmin talk contribs 2,670 bytes +2,670 Created page with "{| class="wikitable" |+ !Material ! ! ! |- | |Freshly isolated granulocytes | | |- | |Phagocytosis buffer (PBS, 0.9 mM CaCl<sub>2</sub>; 0.5 mM MgCl<sub>2</sub>; 5 mM glucose; 1% heat inactivated FCS) | | |- | |5-(6)-carboxyfluorescein succinimidyl ester (FAM SE; CFSE, Fluorescein, FITC) | | |- | |Trypan Blue solution | | |} === Preparations === Two days before experiment: streak out gonococci on selective agar plates and select colony morphology one day before infectio..." Tag: Visual edit
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