Gentamicin Protection Assay: Revision history

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2 June 2025

  • curprev 09:0709:07, 2 June 2025 ESO wikiadmin talk contribs 1,691 bytes +138 No edit summary Tag: Visual edit
  • curprev 09:0409:04, 2 June 2025 ESO wikiadmin talk contribs 1,553 bytes +1,553 Created page with "== Procedure == * Aspirate cell culture medium with a pasteur pipette * Rince attaching cells carefully with cold PBS, aspirate PBS * Add 1.5 mL Trypsin/EDTA and study the detachment of the cells in the microscope * Inactivate Trypsin/EDTA with 3.5 mL medium, transfer the cell suspension into a 15 mL centrifuge tube * Pipette 20 mL of the cell suspension into a Neubauer counting chamber * Centrifuge the remaining cells at 600 rpm for 3 min * During centrifugation: Deter..." Tag: Visual edit
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