GST-Pulldown: Revision history

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27 February 2025

  • curprev 13:1613:16, 27 February 2025 ESO wikiadmin talk contribs 2,554 bytes +2,554 Created page with "{| class="wikitable" |+ !Materials ! |- |GST-Lysis Buffer: |50 mM Tris-HCl (pH 8), 150 mM NaCl, 10% glycerol, 0.05% sodium deoxycholate, 1% Triton- X, 10 µM ZnCl2, 50mM NaF (add protease and phosphatase inhibitors freshly on the day of the experiment) |- |GST-Pulldown/wash buffer: |50 mM Tris-HCl (pH 8), 150 mM NaCl, 10% glycerol, 0.05% sodium deoxycholate, 1% Triton- X, 10 µM ZnCl2, 50mM NaF (add 1mM DTT freshly on the day of the experiment) |- | |Purified recombinant..." Tag: Visual edit
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