FBA-staining of bacteria: Revision history

Jump to navigation Jump to search

Diff selection: Mark the radio buttons of the revisions to compare and hit enter or the button at the bottom.
Legend: (cur) = difference with latest revision, (prev) = difference with preceding revision, m = minor edit.

2 June 2025

  • curprev 09:2809:28, 2 June 2025 ESO wikiadmin talk contribs 1,326 bytes +1,326 Created page with "{{Protokoll-Mix|Inhalt=(5-(6)-carboxyfluorescein succinimidyl ester (CFSE, FAM SE, Fluorescein)<br>NHS-Biotin<br>PBS++ (PBS, 45 nM CaCl 2, 35 nM MgCl 2)<br>4% Paraformaldehyde (PFA)<br>Blocking solution (10% FCS in PBS ++)<br>PBS<br>Streptavidin-AlexaFluor647}} == Procedure == * Dilute 0.2 mg NHS-Biotin in 1 ml PBS (prewarm Biotin to RT before opening the tube) * Collect bacteria from plate in PBS, centrifuge for 4 min, 4000 rpm, and resuspend the pellet in 700 l PB..." Tag: Visual edit
Retrieved from "https://wiki.esonto.de/index.php/FBA-staining_of_bacteria"