Cultivation of HEK293T cells: Revision history

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29 May 2025

15 February 2025

  • curprev 16:2216:22, 15 February 2025 ESO wikiadmin talk contribs 860 bytes +860 Created page with "'''Materials''' Medium: DMEM + 10 % calf serum (CS), PBS, Trypsin / EDTA '''Procedure:''' * Remove growth medium with sterile pasteur pipette from dishes * Wash 1x carefully with PBS, remove PBS * 1 ml Trypsin/EDTA on cells, short incubation (max 2 min) * If cells are detached, stop activity of trypsin with addition of 4 ml medium * Transfer cell solution in falcon tube, centrifugation 800rpm, 3 min, room temperature * Remove medium/ trypsin mixture, add new medium..." Tag: Visual edit
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