Cell viability (MTT Assay): Revision history

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27 February 2025

  • curprev 12:2812:28, 27 February 2025 ESO wikiadmin talk contribs 1,103 bytes +1,103 Created page with "===== Solutions ===== MTT-Solution: 5mg/mL (≙12mM) (toxic and mutagenic) solved in PBS, sterile filtered with 0,2 µM filter Formazan solving solution: isopropanol === Procedure === ----Coat plate before usage For 96 well plates: use 1 - 2x104 cells in 100 µL medium per well and treat the cells according to your experimental setup. The cell number above was optimized for 1 day treatment; for longer incubation periods use fewer cells accordingly. * After treatme..." Tag: Visual edit
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