ESO wikiadmin at 17:50, 15 February 2025

2025-02-15T17:50:51Z

← Older revision		Revision as of 19:50, 15 February 2025
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	# 1 ml medium containing 1~~105~~ or1~~106~~ cells are added into 6 or 24 well plate.		# 1 ml medium containing 110<sup>5</sup> or110<sup>6</sup> cells are added into 6 or 24 well plate.
	# Add 2 µl 1000x Polybrene		# Add 2 µl 1000x Polybrene.
	# Add 1ml of virus supernatant or a corresponding dilution of concentrated virus to the well		# Add 1ml of virus supernatant or a corresponding dilution of concentrated virus to the well.
	# Perform spinfection at 800 g for 1 h at room temperature, disable the brakes of the centrifuge and spin with acceleration = 1 and deceleration = 1.		# Perform spinfection at 800 g for 1 h at room temperature, disable the brakes of the centrifuge and spin with acceleration = 1 and deceleration = 1.
	# Change medium after 1-2 days		# Change medium after 1-2 days.

ESO wikiadmin: Created page with "{| class="wikitable" |+ !Materials ! |- | |Virus containing supernatant |- | |1000x Polybrene (Hexadimethrine bromide) in PBS (8mg/ml) 1106 suspension cells or 1105 adherent cells |- | |24 well plate (for suspension cells) 6 well plate (for adherent cells) |} === Procedure === ---- # 1 ml medium containing 1105 or1106 cells are added into 6 or 24 well plate. # Add 2 µl 1000x Polybrene # Add 1ml of virus supernatant or a corresponding dilution of concentrated virus..."

2025-02-15T17:50:20Z

Created page with "{| class="wikitable" |+ !Materials ! |- | |Virus containing supernatant |- | |1000x Polybrene (Hexadimethrine bromide) in PBS (8mg/ml) 1*106 suspension cells or 1*105 adherent cells |- | |24 well plate (for suspension cells) 6 well plate (for adherent cells) |} === Procedure === ---- # 1 ml medium containing 1*105 or1*106 cells are added into 6 or 24 well plate. # Add 2 µl 1000x Polybrene # Add 1ml of virus supernatant or a corresponding dilution of concentrated virus..."

New page

{| class="wikitable"
|+
!Materials
!
|-
|
|Virus containing supernatant
|-
|
|1000x Polybrene (Hexadimethrine bromide) in PBS (8mg/ml) 1*106 suspension cells or 1*105 adherent cells
|-
|
|24 well plate (for suspension cells) 6 well plate (for adherent cells)
|}

=== Procedure ===
----

# 1 ml medium containing 1*105 or1*106 cells are added into 6 or 24 well plate.
# Add 2 µl 1000x Polybrene
# Add 1ml of virus supernatant or a corresponding dilution of concentrated virus to the well
# Perform spinfection at 800 g for 1 h at room temperature, disable the brakes of the centrifuge and spin with acceleration = 1 and deceleration = 1.
# Change medium after 1-2 days

Viral transduction of mammalian cells via spinfection - Revision history

ESO wikiadmin at 17:50, 15 February 2025