ESO wikiadmin: Created page with "{| class="wikitable" |+ !'''Materials''' |- |siRNA |- |OptiMEM (serum-reduced medium) |- |INTERFERin |} === Procedure === Cells are seeded in 10 cm cell culture plates one day before transfection. 400 µl serum-reduced OptiMEM-medium and 10 µl (20µM) siRNA are mixed for 10 sec with 15 µl INTERFERin and incubated for 10-30 min at room temperature. The cell medium is replaced by fresh cell medium and siRNA preparation is added drop-wise to the cells and incubated for..."

2025-02-15T17:00:23Z

Created page with "{| class="wikitable" |+ !'''Materials''' |- |siRNA |- |OptiMEM (serum-reduced medium) |- |INTERFERin |} === Procedure === Cells are seeded in 10 cm cell culture plates one day before transfection. 400 µl serum-reduced OptiMEM-medium and 10 µl (20µM) siRNA are mixed for 10 sec with 15 µl INTERFERin and incubated for 10-30 min at room temperature. The cell medium is replaced by fresh cell medium and siRNA preparation is added drop-wise to the cells and incubated for..."

New page

{| class="wikitable"
|+
!'''Materials'''
|-
|siRNA
|-
|OptiMEM (serum-reduced medium)
|-
|INTERFERin
|}

=== Procedure ===
Cells are seeded in 10 cm cell culture plates one day before transfection. 400 µl serum-reduced OptiMEM-medium and 10 µl (20µM) siRNA are mixed for 10 sec with 15 µl INTERFERin and incubated for 10-30 min at room temperature. The cell medium is replaced by fresh cell medium and siRNA preparation is added drop-wise to the cells and incubated for 24-72 h.

SiRNA transfection with INTERFERin - Revision history