S1 - Cell culture guidelines - Revision history

ESO wikiadmin at 09:03, 5 June 2025

2025-06-05T09:03:05Z

← Older revision		Revision as of 11:03, 5 June 2025
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	[[File:~~Cellculture meme~~.~~jpg~~\|thumb]]		[[File:Lab Biosafety Level 1.png\|thumb]]

	== '''Getting started:''' ==		== '''Getting started:''' ==

ESO wikiadmin at 09:01, 5 June 2025

2025-06-05T09:01:44Z

ESO wikiadmin at 09:01, 5 June 2025

2025-06-05T09:01:02Z

← Older revision		Revision as of 11:01, 5 June 2025
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	== '''Getting started:''' ==		== '''Getting started:''' ==

	* Turn on hood, open bench  needs several minutes to establish flow, wait for green light		* Turn on hood, open bench ⇒ needs several minutes to establish flow, wait for green light
	* Tie back hair, push back lose sleeves; disinfect hands & arms, put on gloves (can be reused)		* Tie back hair, push back lose sleeves; disinfect hands & arms, put on gloves (can be reused)
	* Wipe down bench and window with 70% EtOH		* Wipe down bench and window with 70% EtOH

ESO wikiadmin at 09:00, 5 June 2025

2025-06-05T09:00:15Z

← Older revision		Revision as of 11:00, 5 June 2025
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	~~'''Getting started~~:~~'''~~		[[File:Cellculture meme.jpg\|thumb]]

	* Turn on hood, open bench  needs several minutes, wait for green light		== '''Getting started:''' ==

			* Turn on hood, open bench  needs several minutes to establish flow, wait for green light
	* Tie back hair, push back lose sleeves; disinfect hands & arms, put on gloves (can be reused)		* Tie back hair, push back lose sleeves; disinfect hands & arms, put on gloves (can be reused)
	* Wipe down bench and window with 70% EtOH		* Wipe down bench and window with 70% EtOH
	* Gather all material you need, spray with 70% EtOH and wipe down before placing under hood		* Gather all material you need, spray with 70% EtOH and wipe down before placing under hood
	* Arrange items under hood towards the back of the bench ~~⇒Keep~~ in mind that you should work a minimum of 15cm inward from the outside edge of the laminar airflow hood; also, leave bottles closed until use and don’t move over open containers; no pouring, use pipettes!		* Arrange items under hood towards the back of the bench → Keep in mind that you should work a minimum of 15cm inward from the outside edge of the laminar airflow hood; also, leave bottles closed until use and don’t move over open containers; no pouring, use pipettes!
	* Always open sterile plastic consumables under the hood		* Always open sterile plastic consumables under the hood
	~~[[File:Cellculture meme.jpg\|thumb]]~~
	'''Before you sit down''' '''& when you are done''': If necessary		== '''Before you sit down''' '''& when you are done''': If necessary ==

	* Stock up pipettes and falcons (also glass pasteur pipettes)		* Stock up pipettes and falcons (also glass pasteur pipettes)
	* Refill EtOH bottles (also canister, if empty)		* Refill EtOH bottles (also canister, if empty)
	* Check vacuum bottles		* Check vacuum bottles
	* Turn vacuum on or off, if applicable		* Turn vacuum on or off, if applicable

	'''General lab maintenance/ behaviour:'''		== '''General lab maintenance/ behaviour:''' ==

	* Clean spills immediately		* Minimize waste whenever possible, plan experiments accordingly
	* ~~Minimize~~ waste~~: Think ahead~~ and ~~plan experiment accordingly~~		* Clean spills immediately with 70% EtOH
			* Let appropriate person know if things are running low: e.g. Jan for plastic consumables, Marleen for PFA, Claudia/Susi for Trypsin, FCS/CS etc., Steffi for general S1 issues...
			* Thaw new trypsin bottle for next person if current bottle is close to empty
			* Fill up glass pasteur pipettes if you emptied a canister  stick on “heat-dry” tape & put on cart
			* Empty trash can if ¾ full, lightly close with tape & place on cart for autoclaving  use 2 new autoclave bags!
			* Collect polystyrene waste (f.ex. pipets w/o filter, cell culture dishes and multiwell plates) in SEPARATE trash can
			* Make sure clip on vacuum hose is on completely –> or else pump keeps running!
	* Plug in pipette boy if battery is low		* Plug in pipette boy if battery is low
	* Thaw new trypsin bottle if close to end
	* Let appropriate person know if things are running low: e.g. Jan for plastic consumables, Marleen for PFA, Claudia/Susi for Trypsin, serum etc., Steffi for general S1 issues...
	* Fill up glass pasteur pipettes if you emptied a canister
	* Collect polystyrene lab waste (f.ex. pipets w/o filter, cell culture dishes and multiwell plates) in separate trash can
	* Empty trash can if full, place on cart for autoclaving
	* Make sure clip-on vacuum hose is on completely –> or else pump keeps running!
	* Close bench when you step away for an extended period („stand-by mode“)		* Close bench when you step away for an extended period („stand-by mode“)
	* Vent vacuum when you are done (but it‘s not the end of the day yet)		* Vent vacuum when you are done (but it‘s not the end of the day yet)

ESO wikiadmin at 15:26, 3 June 2025

2025-06-03T15:26:07Z

← Older revision		Revision as of 17:26, 3 June 2025
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	* If fluorescence lamp was used, set timer for 30min  Should not be switched back on again within 30min!		* If fluorescence lamp was used, set timer for 30min  Should not be switched back on again within 30min!
	* Turn on UV light under bench for 30min if a contamination is suspected! (set timer for 30min)		* Turn on UV light under bench for 30min if a contamination is suspected! (set timer for 30min)
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ESO wikiadmin at 15:24, 3 June 2025

2025-06-03T15:24:32Z

← Older revision		Revision as of 17:24, 3 June 2025
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	* Arrange items under hood towards the back of the bench ⇒Keep in mind that you should work a minimum of 15cm inward from the outside edge of the laminar airflow hood; also, leave bottles closed until use and don’t move over open containers; no pouring, use pipettes!		* Arrange items under hood towards the back of the bench ⇒Keep in mind that you should work a minimum of 15cm inward from the outside edge of the laminar airflow hood; also, leave bottles closed until use and don’t move over open containers; no pouring, use pipettes!
	* Always open sterile plastic consumables under the hood		* Always open sterile plastic consumables under the hood
			[[File:Cellculture meme.jpg\|thumb]]
	'''Before you sit down''' '''& when you are done''': If necessary		'''Before you sit down''' '''& when you are done''': If necessary

	* Stock up pipettes and falcons (also glass pasteur pipettes)		* Stock up pipettes and falcons (also glass pasteur pipettes)
	* Refill EtOH bottles (also canister, if empty)		* Refill EtOH bottles (also canister, if empty)

ESO wikiadmin: Created page with "'''Getting started:''' * Turn on hood, open bench  needs several minutes, wait for green light * Tie back hair, push back lose sleeves; disinfect hands & arms, put on gloves (can be reused) * Wipe down bench and window with 70% EtOH * Gather all material you need, spray with 70% EtOH and wipe down before placing under hood * Arrange items under hood towards the back of the bench ⇒Keep in mind that you should work a minimum of 15cm inward from the outside edge of th..."

2025-06-03T15:22:27Z

Created page with "'''Getting started:''' * Turn on hood, open bench  needs several minutes, wait for green light * Tie back hair, push back lose sleeves; disinfect hands & arms, put on gloves (can be reused) * Wipe down bench and window with 70% EtOH * Gather all material you need, spray with 70% EtOH and wipe down before placing under hood * Arrange items under hood towards the back of the bench ⇒Keep in mind that you should work a minimum of 15cm inward from the outside edge of th..."

New page

'''Getting started:'''

* Turn on hood, open bench  needs several minutes, wait for green light
* Tie back hair, push back lose sleeves; disinfect hands & arms, put on gloves (can be reused)
* Wipe down bench and window with 70% EtOH
* Gather all material you need, spray with 70% EtOH and wipe down before placing under hood
* Arrange items under hood towards the back of the bench ⇒Keep in mind that you should work a minimum of 15cm inward from the outside edge of the laminar airflow hood; also, leave bottles closed until use and don’t move over open containers; no pouring, use pipettes!
* Always open sterile plastic consumables under the hood

'''Before you sit down''' '''& when you are done''': If necessary

* Stock up pipettes and falcons (also glass pasteur pipettes)
* Refill EtOH bottles (also canister, if empty)
* Check vacuum bottles
* Turn vacuum on or off, if applicable

'''General lab maintenance/ behaviour:'''

* Clean spills immediately
* Minimize waste: Think ahead and plan experiment accordingly
* Plug in pipette boy if battery is low
* Thaw new trypsin bottle if close to end
* Let appropriate person know if things are running low: e.g. Jan for plastic consumables, Marleen for PFA, Claudia/Susi for Trypsin, serum etc., Steffi for general S1 issues...
* Fill up glass pasteur pipettes if you emptied a canister
* Collect polystyrene lab waste (f.ex. pipets w/o filter, cell culture dishes and multiwell plates) in separate trash can
* Empty trash can if full, place on cart for autoclaving
* Make sure clip-on vacuum hose is on completely –> or else pump keeps running!
* Close bench when you step away for an extended period („stand-by mode“)
* Vent vacuum when you are done (but it‘s not the end of the day yet)
* Turn off computer AND monitor when done using, turn of microscope light when done using
* If fluorescence lamp was used, set timer for 30min  Should not be switched back on again within 30min!
* Turn on UV light under bench for 30min if a contamination is suspected! (set timer for 30min)