Lab Etiquette (ENG) - Revision history

ESO wikiadmin: /* Lab Book */

2025-06-16T09:19:50Z

Lab Book

← Older revision		Revision as of 11:19, 16 June 2025
Line 57:		Line 57:
	* In vitro Kinase Assay: "Erik"		* In vitro Kinase Assay: "Erik"
	* [[Immunoprecipitation]] (MHE)		* [[Immunoprecipitation]] (MHE)
	* Protein expression and purification~~: "Sarah"~~		* [[Protein expression and purification]] (SBA)
	* Lentivirus production: "Marlene"		* Lentivirus production: "Marlene"
	* Bacterial growth curve: "Ann-Kathrin"		* Bacterial growth curve: "Ann-Kathrin"

ESO wikiadmin at 09:19, 16 June 2025

2025-06-16T09:19:25Z

← Older revision		Revision as of 11:19, 16 June 2025
Line 56:		Line 56:
	* Cell transfection: "HEKcell-transfection-Protocol-JWK.pdf"		* Cell transfection: "HEKcell-transfection-Protocol-JWK.pdf"
	* In vitro Kinase Assay: "Erik"		* In vitro Kinase Assay: "Erik"
	* Immunoprecipitation~~: "Marleen"~~		* [[Immunoprecipitation]] (MHE)
	* Protein expression and purification: "Sarah"		* Protein expression and purification: "Sarah"
	* Lentivirus production: "Marlene"		* Lentivirus production: "Marlene"

ESO wikiadmin at 12:20, 5 June 2025

2025-06-05T12:20:20Z

← Older revision		Revision as of 14:20, 5 June 2025
Line 110:		Line 110:

	'''Thesis:''' for your thesis: for '''every''' figure in your thesis create a subfolder named with the Figure-Number and include in each subfolder both: the final figure as pdf- and illustrator-file and the '''used raw data''' (subdivided in panels A, B, etc.). The name of the raw data file should remain the same as it is found in your DATA-folder and lab book)		'''Thesis:''' for your thesis: for '''every''' figure in your thesis create a subfolder named with the Figure-Number and include in each subfolder both: the final figure as pdf- and illustrator-file and the '''used raw data''' (subdivided in panels A, B, etc.). The name of the raw data file should remain the same as it is found in your DATA-folder and lab book)
	[[File:Labetiquette fileserver.jpg\|thumb]]		[[File:Labetiquette fileserver.jpg\|thumb\|center\|696x696px\|'''Fig. 1:''' Example of the user folder structure on the Fileserver_LSH_Data of user UE_Universal_Example.]]

	== Laboratory Workspace ==		== Laboratory Workspace ==

ESO wikiadmin at 12:19, 5 June 2025

2025-06-05T12:19:31Z

← Older revision		Revision as of 14:19, 5 June 2025
Line 110:		Line 110:

	'''Thesis:''' for your thesis: for '''every''' figure in your thesis create a subfolder named with the Figure-Number and include in each subfolder both: the final figure as pdf- and illustrator-file and the '''used raw data''' (subdivided in panels A, B, etc.). The name of the raw data file should remain the same as it is found in your DATA-folder and lab book)		'''Thesis:''' for your thesis: for '''every''' figure in your thesis create a subfolder named with the Figure-Number and include in each subfolder both: the final figure as pdf- and illustrator-file and the '''used raw data''' (subdivided in panels A, B, etc.). The name of the raw data file should remain the same as it is found in your DATA-folder and lab book)
			[[File:Labetiquette fileserver.jpg\|thumb]]

	== Laboratory Workspace ==		== Laboratory Workspace ==

ESO wikiadmin at 11:57, 5 June 2025

2025-06-05T11:57:21Z

← Older revision		Revision as of 13:57, 5 June 2025
Line 1:		Line 1:
	'''Diese Seite ist auch auf [[Lab Etiquette\|Deutsch]] verfügbar.'''<div style="border: 1px solid #ccc; background: #f9f9f9; padding: 1em; margin-bottom: 1em;">		'''Diese Seite ist auch auf [[Lab Etiquette\|Deutsch]] 🇩🇪 verfügbar.'''<div style="border: 1px solid #ccc; background: #f9f9f9; padding: 1em; margin-bottom: 1em;">
	'''<span style="font-size:120%; font-weight:bold;">Welcome to the lab – think together, act together!</span>'''		'''<span style="font-size:120%; font-weight:bold;">Welcome to the lab – think together, act together!</span>'''

ESO wikiadmin: /* Laboratory Safety */

2025-06-05T09:13:08Z

Laboratory Safety

← Older revision		Revision as of 11:13, 5 June 2025
Line 191:		Line 191:

	== Laboratory Safety ==		== Laboratory Safety ==
	See ~~<nowiki>~~[[All About Safety\|Safety Guidelines]]~~</nowiki>~~		See [[All About Safety\|Safety Guidelines]].

ESO wikiadmin at 09:11, 5 June 2025

2025-06-05T09:11:40Z

Show changes

ESO wikiadmin at 14:48, 7 May 2025

2025-05-07T14:48:41Z

← Older revision		Revision as of 16:48, 7 May 2025
Line 1:		Line 1:
	'''Diese Seite ist auch auf [[~~Laborfibel~~\|Deutsch]] verfügbar.'''<div style="border: 1px solid #ccc; background: #f9f9f9; padding: 1em; margin-bottom: 1em;">		'''Diese Seite ist auch auf [[Lab Etiquette\|Deutsch]] verfügbar.'''<div style="border: 1px solid #ccc; background: #f9f9f9; padding: 1em; margin-bottom: 1em;">
	'''<span style="font-size:120%; font-weight:bold;">Welcome to the lab – think together, act together!</span>'''		'''<span style="font-size:120%; font-weight:bold;">Welcome to the lab – think together, act together!</span>'''

ESO wikiadmin at 14:47, 7 May 2025

2025-05-07T14:47:49Z

← Older revision		Revision as of 16:47, 7 May 2025
Line 1:		Line 1:
	'''<div style="border: 1px solid #ccc; background: #f9f9f9; padding: 1em; margin-bottom: 1em;">		'''Diese Seite ist auch auf [[Laborfibel\|Deutsch]] verfügbar.'''<div style="border: 1px solid #ccc; background: #f9f9f9; padding: 1em; margin-bottom: 1em;">
	'''<span style="font-size:120%; font-weight:bold;">Welcome to the lab – think together, act together!</span>'''		'''<span style="font-size:120%; font-weight:bold;">Welcome to the lab – think together, act together!</span>'''

	A well-functioning lab doesn’t rely solely on rules but on mutual awareness and shared responsibility. Especially for newcomers, it’s better to ask one question too many than to do the wrong thing silently. If you notice that something is running out – let people know. If you change something, inform others. That’s how we keep things smooth, safe, and effective.		A well-functioning lab doesn’t rely solely on rules but on mutual awareness and shared responsibility. Especially for newcomers, it’s better to ask one question too many than to do the wrong thing silently. If you notice that something is running out – let people know. If you change something, inform others. That’s how we keep things smooth, safe, and effective.
			</div>

	~~</div>'''~~		== Lab Etiquette – Common Information ==

	~~\== Lab Etiquette – Common Information ==~~		=== Computer Use ===

	\=== Computer Use ===
	There is a "Public Computer" available in the office area for general use. Please ensure it is always accessible for work-related purposes. Personal laptops may be connected to the LAN if they have '''an active virus scanner and firewall'''. The system administrator (ESO, CRH) provides support and access to the internal file server.		There is a "Public Computer" available in the office area for general use. Please ensure it is always accessible for work-related purposes. Personal laptops may be connected to the LAN if they have '''an active virus scanner and firewall'''. The system administrator (ESO, CRH) provides support and access to the internal file server.

	The file server ("~~Fileserver~~\~~_Data~~") provides access to several programs, including ImageLab, MS Office, Leica File Browser, Adobe Photoshop, Adobe Acrobat, Adobe Illustrator, Clone Manager, Endnote 8.0, GraphPad Prism 5.		The file server (\"Fileserver_Data\") provides access to several programs, including ImageLab, MS Office, Leica File Browser, Adobe Photoshop, Adobe Acrobat, Adobe Illustrator, Clone Manager, Endnote 8.0, GraphPad Prism 5.

	To ensure smooth collaboration, please use these software versions for all lab-related work.		To ensure smooth collaboration, please use these software versions for all lab-related work.

	\=== Initials & Labeling ===		=== Initials & Labeling ===
	Every lab member receives a unique set of '''initials''' via the secretary (e.g., "ESO" for Erik Sontowski). These must be used to label '''all samples, solutions, etc.''' The list is stored at: <code>/~~AG\_Hauck~~/Labororganisation/Namenskuerzel.doc</code>		Every lab member receives a unique set of '''initials''' via the secretary (e.g., "ESO" for Erik Sontowski). These must be used to label '''all samples, solutions, etc.''' The list is stored at: <code>/AG_Hauck/Labororganisation/Namenskuerzel.doc</code>

	\=== Lab Workspaces ===		=== Lab Workspaces ===
	Workspaces are either general or assigned. Do not use private desks without asking. Shared work areas (e.g. cell culture, microscopy, electrophoresis) must be '''cleaned after use'''.		Workspaces are either general or assigned. Do not use private desks without asking. Shared work areas (e.g. cell culture, microscopy, electrophoresis) must be '''cleaned after use'''.

Line 31:		Line 30:
	* Refrigerated (4 °C), frozen (-20 °C), and ultra-cold (-80 °C)		* Refrigerated (4 °C), frozen (-20 °C), and ultra-cold (-80 °C)
	* Only use labeled boxes for long-term -80°C storage		* Only use labeled boxes for long-term -80°C storage
	* Include a datasheet per box in the -80°C binder (<code>/~~AG\_Hauck~~/Vorlagen/-80-Ordner.doc</code>)		* Include a datasheet per box in the -80°C binder (<code>/AG_Hauck/Vorlagen/-80-Ordner.doc</code>)

	Minimize time spent with freezers open – know what you need before opening!		Minimize time spent with freezers open – know what you need before opening!

	\=== Lab Book ===		=== Lab Book ===
	All lab members (including students) must maintain a lab book (provided by the secretary), which documents work in '''chronological order'''.		All lab members (including students) must maintain a lab book (provided by the secretary), which documents work in '''chronological order'''.

Line 43:		Line 42:
	* Example protocols available at:		* Example protocols available at:

	* <code>/~~AG\_Hauck~~/Vorlagen/Protokoll-Beispiel-Genta.pdf</code>		* <code>/AG_Hauck/Vorlagen/Protokoll-Beispiel-Genta.pdf</code>
	* <code>/Protokoll-Beispiel-PCR.pdf</code>		* <code>/Protokoll-Beispiel-PCR.pdf</code>
	* <code>/Protokoll-Beispiel-Transfektion-Lysate.pdf</code>		* <code>/Protokoll-Beispiel-Transfektion-Lysate.pdf</code>

	For digital results (e.g. microscopy, FACS), attach a separate sample protocol: <code>/Vorlagen/Probenprotokoll.doc</code>		For digital results (e.g. microscopy, FACS), attach a separate sample protocol: <code>/Vorlagen/Probenprotokoll.doc</code>

	\=== Lab Protocols ===		=== Lab Protocols ===
	Many standard experiments are documented in shared protocols (<code>/Labororganisation/Protokolle.doc</code>, VTK scripts). Stick to these when possible; note any deviations in your lab book. Reagents & solutions are listed under <code>/Chemikalien/Lösungen</code>.		Many standard experiments are documented in shared protocols (<code>/Labororganisation/Protokolle.doc</code>, VTK scripts). Stick to these when possible; note any deviations in your lab book. Reagents & solutions are listed under <code>/Chemikalien/Lösungen</code>.

	\=== Lab Jobs ===		=== Lab Jobs ===
	Every lab member (students included) is assigned a Lab Job (e.g. solution prep, equipment care). Responsibilities are posted on the "Verantwortlichkeiten" list (whiteboard & file server).		Every lab member (students included) is assigned a Lab Job (e.g. solution prep, equipment care). Responsibilities are posted on the "Verantwortlichkeiten" list (whiteboard & file server).

	\=== Everyday Responsibility ===		=== Everyday Responsibility ===
	You help keep the lab functional by:		You help keep the lab functional by:

Line 64:		Line 62:
	* placing autoclave items in front of cell culture lab		* placing autoclave items in front of cell culture lab

	\=== Chemicals & Solutions ===		=== Chemicals & Solutions ===
	Many solutions are prepared centrally. For personal use:		Many solutions are prepared centrally. For personal use:

Line 76:		Line 74:
	* and in the "Protocols and Methods" wiki section		* and in the "Protocols and Methods" wiki section

	\=== Shared Collections ===		=== Shared Collections ===
	We maintain several shared biological/chemical collections:		We maintain several shared biological/chemical collections:

Line 89:		Line 87:
	Documentation folders available near the public computer in the office hallway.		Documentation folders available near the public computer in the office hallway.

	\=== Competent Cells ===		=== Competent Cells ===
	Aliquots (200 µl) are stored at -80 °C near ML641. Retrieve them '''quickly and efficiently'''. Strain overview ▶ here (wiki link).		Aliquots (200 µl) are stored at -80 °C near ML641. Retrieve them '''quickly and efficiently'''. Strain overview ▶ here (wiki link).

	\=== Enzymes ===		=== Enzymes ===
	Shared use requires extra care:		Shared use requires extra care:

Line 99:		Line 97:
	* Avoid skin contact with tube lids		* Avoid skin contact with tube lids

	\=== Orders ===		=== Orders ===
	Report low stock early! For common supplies: whiteboard near cell culture or notify the TAs. For project-specific materials: consult CRH before ordering.		Report low stock early! For common supplies: whiteboard near cell culture or notify the TAs. For project-specific materials: consult CRH before ordering.

Line 106:		Line 104:
	Deliveries must be checked, signed, and submitted to PS. Datasheets go into the appropriate folders, labeled and dated.		Deliveries must be checked, signed, and submitted to PS. Datasheets go into the appropriate folders, labeled and dated.

	\=== Seminars & Meetings ===		=== Seminars & Meetings ===

	* Monday 08:45: general lab meeting + project presentations		* Monday 08:45: general lab meeting + project presentations
Line 113:		Line 111:
	* Project meetings by arrangement with CRH – lab book required		* Project meetings by arrangement with CRH – lab book required

	\=== Working Hours & Leave ===		=== Working Hours & Leave ===

	* Non-scientific staff: use official timecards		* Non-scientific staff: use official timecards
Line 121:		Line 119:
	* Students: coordinate absences with CRH/supervisor		* Students: coordinate absences with CRH/supervisor

	\=== Illness ===		=== Illness ===
	Notify the secretary ASAP in case of illness. A doctor’s note is required from day 3 onward.		Notify the secretary ASAP in case of illness. A doctor’s note is required from day 3 onward.

	~~---~~

	~~<small>This version is a revised and structured edition of the internal lab etiquette guide for the Department of Cell Biology, formatted for use in the internal wiki.</small>~~

ESO wikiadmin: Created page with "'''
'''Welcome to the lab – think together, act together!''' A well-functioning lab doesn’t rely solely on rules but on mutual awareness and shared responsibility. Especially for newcomers, it’s better to ask one question too many than to do the wrong thing silently. If you notice that something is running out – let p..."

2025-05-07T14:45:25Z

Created page with "'''<div style="border: 1px solid #ccc; background: #f9f9f9; padding: 1em; margin-bottom: 1em;"> '''<span style="font-size:120%; font-weight:bold;">Welcome to the lab – think together, act together!</span>''' A well-functioning lab doesn’t rely solely on rules but on mutual awareness and shared responsibility. Especially for newcomers, it’s better to ask one question too many than to do the wrong thing silently. If you notice that something is running out – let p..."

New page

'''<div style="border: 1px solid #ccc; background: #f9f9f9; padding: 1em; margin-bottom: 1em;">
'''<span style="font-size:120%; font-weight:bold;">Welcome to the lab – think together, act together!</span>'''

A well-functioning lab doesn’t rely solely on rules but on mutual awareness and shared responsibility. Especially for newcomers, it’s better to ask one question too many than to do the wrong thing silently. If you notice that something is running out – let people know. If you change something, inform others. That’s how we keep things smooth, safe, and effective.

</div>'''

\== Lab Etiquette – Common Information ==

\=== Computer Use ===
There is a "Public Computer" available in the office area for general use. Please ensure it is always accessible for work-related purposes. Personal laptops may be connected to the LAN if they have '''an active virus scanner and firewall'''. The system administrator (ESO, CRH) provides support and access to the internal file server.

The file server ("Fileserver\_Data") provides access to several programs, including ImageLab, MS Office, Leica File Browser, Adobe Photoshop, Adobe Acrobat, Adobe Illustrator, Clone Manager, Endnote 8.0, GraphPad Prism 5.

To ensure smooth collaboration, please use these software versions for all lab-related work.

\=== Initials & Labeling ===
Every lab member receives a unique set of '''initials''' via the secretary (e.g., "ESO" for Erik Sontowski). These must be used to label '''all samples, solutions, etc.''' The list is stored at: <code>/AG\_Hauck/Labororganisation/Namenskuerzel.doc</code>

\=== Lab Workspaces ===
Workspaces are either general or assigned. Do not use private desks without asking. Shared work areas (e.g. cell culture, microscopy, electrophoresis) must be '''cleaned after use'''.

'''Each person is responsible for:'''

* keeping their space clean (including shelves/drawers)
* disinfecting the bench at least once a week
* checking stored samples regularly

'''Sample Storage:'''

* Refrigerated (4 °C), frozen (-20 °C), and ultra-cold (-80 °C)
* Only use labeled boxes for long-term -80°C storage
* Include a datasheet per box in the -80°C binder (<code>/AG\_Hauck/Vorlagen/-80-Ordner.doc</code>)

Minimize time spent with freezers open – know what you need before opening!

\=== Lab Book ===
All lab members (including students) must maintain a lab book (provided by the secretary), which documents work in '''chronological order'''.

* Pages must be numbered
* Create a table of contents on the first pages
* Original printouts (e.g. gels) must be glued/taped in
* Example protocols available at:

* <code>/AG\_Hauck/Vorlagen/Protokoll-Beispiel-Genta.pdf</code>
* <code>/Protokoll-Beispiel-PCR.pdf</code>
* <code>/Protokoll-Beispiel-Transfektion-Lysate.pdf</code>

For digital results (e.g. microscopy, FACS), attach a separate sample protocol: <code>/Vorlagen/Probenprotokoll.doc</code>

\=== Lab Protocols ===
Many standard experiments are documented in shared protocols (<code>/Labororganisation/Protokolle.doc</code>, VTK scripts). Stick to these when possible; note any deviations in your lab book. Reagents & solutions are listed under <code>/Chemikalien/Lösungen</code>.

\=== Lab Jobs ===
Every lab member (students included) is assigned a Lab Job (e.g. solution prep, equipment care). Responsibilities are posted on the "Verantwortlichkeiten" list (whiteboard & file server).

\=== Everyday Responsibility ===
You help keep the lab functional by:

* notifying staff when supplies run low
* disposing of waste properly (contaminated, cardboard, etc.)
* keeping common areas clean (tea kitchen, common room)
* restocking pipette tips, tubes, etc.
* placing autoclave items in front of cell culture lab

\=== Chemicals & Solutions ===
Many solutions are prepared centrally. For personal use:

* avoid preparing excess amounts
* label properly: initials, date, name, concentration, hazard symbol

Recipes can be found:

* as a printed booklet in the lab
* digitally: <code>/Labororganisation/Rezepte.doc</code>
* and in the "Protocols and Methods" wiki section

\=== Shared Collections ===
We maintain several shared biological/chemical collections:

* '''Strain Collection''': all bacteria (non-/pathogenic); stored at -80 °C, numbered by SF
* '''Plasmids''': stored at 4 °C, follow strain numbers
* '''Cell lines''': cryopreserved, managed by SF & CH
* '''Viruses''': at -80 °C, MS Access overview
* '''Oligos''': in TE buffer, -20 °C, ordered via JWK
* '''Antibodies''': primary/secondary, MS Access database, organized by CH & SF
* '''Proteins''', '''Inhibitors''', '''Peptides''': documented & structured

Documentation folders available near the public computer in the office hallway.

\=== Competent Cells ===
Aliquots (200 µl) are stored at -80 °C near ML641. Retrieve them '''quickly and efficiently'''. Strain overview ▶ here (wiki link).

\=== Enzymes ===
Shared use requires extra care:

* Use filter tips
* Handle only with chilled paraffin block
* Avoid skin contact with tube lids

\=== Orders ===
Report low stock early! For common supplies: whiteboard near cell culture or notify the TAs. For project-specific materials: consult CRH before ordering.

Sequencing: use prepaid LGC Genomics / Azenta labels with supervisor approval. Limit to one reaction per construct unless approved.

Deliveries must be checked, signed, and submitted to PS. Datasheets go into the appropriate folders, labeled and dated.

\=== Seminars & Meetings ===

* Monday 08:45: general lab meeting + project presentations
* Wednesday (biweekly), 16:00: Journal Club (paper shared by PDF)
* Thursday 12:15: Department Seminar (optional)
* Project meetings by arrangement with CRH – lab book required

\=== Working Hours & Leave ===

* Non-scientific staff: use official timecards
* Scientific staff: present during core hours (9–12 & 14–16)
* Leave via: personal.uni-konstanz.de – always coordinate with CRH
* Ensure sample/culture care & Lab Job coverage during absence
* Students: coordinate absences with CRH/supervisor

\=== Illness ===
Notify the secretary ASAP in case of illness. A doctor’s note is required from day 3 onward.

---

<small>This version is a revised and structured edition of the internal lab etiquette guide for the Department of Cell Biology, formatted for use in the internal wiki.</small>