ESO wikiadmin: Created page with "=== Procedure === 1x10⁶ granulocytes are infected with bacteria (MOI 20) for 15 min at 37°C under rotation. Phagocytosis is stopped by the addition of 500 µl of ice cold PBS. Samples are centrifuged at 4°C for 5 min at 200 g and washed once with PBS. After centrifugation samples are taken up in PBS or SDS-buffer."

2025-02-15T17:12:48Z

Created page with "=== Procedure === 1x10<sup>6</sup> granulocytes are infected with bacteria (MOI 20) for 15 min at 37°C under rotation. Phagocytosis is stopped by the addition of 500 µl of ice cold PBS. Samples are centrifuged at 4°C for 5 min at 200 g and washed once with PBS. After centrifugation samples are taken up in PBS or SDS-buffer."

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=== Procedure ===
1x10<sup>6</sup> granulocytes are infected with bacteria (MOI 20) for 15 min at 37°C under rotation. Phagocytosis is stopped by the addition of 500 µl of ice cold PBS. Samples are centrifuged at 4°C for 5 min at 200 g and washed once with PBS. After centrifugation samples are taken up in PBS or SDS-buffer.

Infection of granulocytes - Revision history