ESO wikiadmin: Created page with "{| class="wikitable" |+ !Materials ! |- |Staining Solution |25% (v/v) isopropanol 10% (v/v) acetic acid 0.03% (w/v) Coomassie Brillant Blue R250 |- |Destaining Sol. |500 mL methanol; 500 mL dH₂O 100 mL acetic acid |} === Procedure === ----The gel is heated in Coomassie staining solution briefly in the microwave and incubated for 30 min on a shaker. After this time it is incubated in destaining solution, so that the blue protein bands are visible against a cle..."

2025-02-27T13:15:21Z

Created page with "{| class="wikitable" |+ !Materials ! |- |Staining Solution |25% (v/v) isopropanol 10% (v/v) acetic acid 0.03% (w/v) Coomassie Brillant Blue R250 |- |Destaining Sol. |500 mL methanol; 500 mL dH<sub>2</sub>O 100 mL acetic acid |} === Procedure === ----The gel is heated in Coomassie staining solution briefly in the microwave and incubated for 30 min on a shaker. After this time it is incubated in destaining solution, so that the blue protein bands are visible against a cle..."

New page

{| class="wikitable"
|+
!Materials
!
|-
|Staining Solution
|25% (v/v) isopropanol
10% (v/v) acetic acid
0.03% (w/v) Coomassie Brillant Blue R250
|-
|Destaining Sol.
|500 mL methanol; 500 mL dH<sub>2</sub>O
100 mL acetic acid
|}

=== Procedure ===
----The gel is heated in Coomassie staining solution briefly in the microwave and incubated for 30 min on a shaker. After this time it is incubated in destaining solution, so that the blue protein bands are visible against a clear background.

Coomassie Blue staining of gels - Revision history