ESO wikiadmin at 14:47, 30 May 2025

2025-05-30T14:47:33Z

← Older revision		Revision as of 16:47, 30 May 2025
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ESO wikiadmin: Created page with "{| class="wikitable" |+ !Materials ! ! ! |- | |Virus containing supernatant | | |- | |20% sucrose in PBS | | |- | |1% BSA in PBS | | |} === Procedure === ---- # 4 ml of 20% sucrose solution are added to an ultracentrifuge tube and overlayed with 26 ml of filtrated virus containing supernatant # the tubes are placed in aerosol tight swing buckets # centrifugation is carried at 20.000 rpm , 4°C for 2h15min (Rotor: SW32Ti) # after discarding the supernatant the pellet is..."

2025-02-15T17:52:33Z

Created page with "{| class="wikitable" |+ !Materials ! ! ! |- | |Virus containing supernatant | | |- | |20% sucrose in PBS | | |- | |1% BSA in PBS | | |} === Procedure === ---- # 4 ml of 20% sucrose solution are added to an ultracentrifuge tube and overlayed with 26 ml of filtrated virus containing supernatant # the tubes are placed in aerosol tight swing buckets # centrifugation is carried at 20.000 rpm , 4°C for 2h15min (Rotor: SW32Ti) # after discarding the supernatant the pellet is..."

New page

{| class="wikitable"
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!Materials
!
!
!
|-
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|Virus containing supernatant
|
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|-
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|20% sucrose in PBS
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|-
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|1% BSA in PBS
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|}

=== Procedure ===
----

# 4 ml of 20% sucrose solution are added to an ultracentrifuge tube and overlayed with 26 ml of filtrated virus containing supernatant
# the tubes are placed in aerosol tight swing buckets
# centrifugation is carried at 20.000 rpm , 4°C for 2h15min (Rotor: SW32Ti)
# after discarding the supernatant the pellet is resuspended in 250µl 1%BSA
# Aliquot and freeze in liquid nitrogen
# Store at -80°C (repeated freezing and thawing might result in titer reduction)

Concentration of lentiviral particles via ultracentrifugation - Revision history

ESO wikiadmin at 14:47, 30 May 2025