All About Safety - Revision history

ESO wikiadmin at 11:58, 5 June 2025

2025-06-05T11:58:53Z

← Older revision		Revision as of 13:58, 5 June 2025
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	== Training by Lab Supervisors ==		== Training by Lab Supervisors ==
	Cell Culture – Any new member must pass the introduction to the cell culture laboratory by SSJ before any work is carried out there. ~~Weitere Informationen unter~~ [[S1 - Cell culture guidelines\|'''S1 - Cell culture guidelines''']]		Cell Culture – Any new member must pass the introduction to the cell culture laboratory by SSJ before any work is carried out there. Further information here: [[S1 - Cell culture guidelines\|'''S1 - Cell culture guidelines''']]

	Fluorescence Microscope: xxx		Fluorescence Microscope: xxx

ESO wikiadmin at 11:58, 5 June 2025

2025-06-05T11:58:33Z

← Older revision		Revision as of 13:58, 5 June 2025
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	== Training by Lab Supervisors ==		== Training by Lab Supervisors ==
	Cell Culture – Any new member must pass the introduction to the cell culture laboratory by SSJ before any work is carried out there. Weitere Informationen unter [[S1 - Cell culture guidelines]]		Cell Culture – Any new member must pass the introduction to the cell culture laboratory by SSJ before any work is carried out there. Weitere Informationen unter [[S1 - Cell culture guidelines\|'''S1 - Cell culture guidelines''']]

	Fluorescence Microscope: xxx		Fluorescence Microscope: xxx

ESO wikiadmin at 15:19, 3 June 2025

2025-06-03T15:19:55Z

← Older revision		Revision as of 17:19, 3 June 2025
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	== Training by Lab Supervisors ==		== Training by Lab Supervisors ==
	~~Working in~~ the Cell ~~Culture Lab~~		Cell Culture – Any new member must pass the introduction to the cell culture laboratory by SSJ before any work is carried out there. Weitere Informationen unter [[S1 - Cell culture guidelines]]

	Fluorescence Microscope: xxx		Fluorescence Microscope: xxx

ESO wikiadmin at 12:32, 3 June 2025

2025-06-03T12:32:41Z

← Older revision		Revision as of 14:32, 3 June 2025
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	== Sharp Objects ==		== Sharp Objects ==
	~~• ‘’‘Needles~~/Razor Blades/~~Scalpels’’’~~ Exercise caution when working with these tools due to injury risk. Do NOT re-cap used needles—dispose of them immediately. These items must be discarded in clearly labeled yellow plastic containers (single-use). One container is located in the cold room, another under Fume Hood II.
	~~• ‘’‘Pasteur~~ Pipettes’’’ Pasteur pipettes should be collected in large, yellow plastic containers (reusable), labeled “Nur Pasteurpipetten / Pasteur Pipettes ONLY”. One container is located in each cell culture lab and one in the cold room. Please DO NOT dispose of other sharp items (scalpels, razor blades, needles, etc.) in these containers, as the contents will be autoclaved and then treated as glass waste.		* Needles/Razor Blades/Scalpels Exercise caution when working with these tools due to injury risk. Do NOT re-cap used needles—dispose of them immediately. These items must be discarded in clearly labeled yellow plastic containers (single-use). One container is located in the cold room, another under Fume Hood II.
	~~• ‘’‘Glass~~ Bottles/Broken Glass’’’ Broken glass and empty lab glass bottles should be disposed of in the designated containers.		* Pasteur Pipettes’’’ Pasteur pipettes should be collected in large, yellow plastic containers (reusable), labeled “Nur Pasteurpipetten / Pasteur Pipettes ONLY”. One container is located in each cell culture lab and one in the cold room. Please DO NOT dispose of other sharp items (scalpels, razor blades, needles, etc.) in these containers, as the contents will be autoclaved and then treated as glass waste.
			* Glass Bottles/Broken Glass’’’ Broken glass and empty lab glass bottles should be disposed of in the designated containers.

	== Training by Lab Supervisors ==		== Training by Lab Supervisors ==
	~~‘’‘Working~~ in the Cell Culture ~~Lab’’’~~		Working in the Cell Culture Lab

	Fluorescence Microscope: xxx		Fluorescence Microscope: xxx

	~~‘’‘Working~~ in the S2 Lab (Infection Lab)~~’’’~~		Working in the S2 Lab (Infection Lab)

	xxx		xxx

	~~‘’‘Working~~ at the RNA ~~Workstation’’’~~		Working at the RNA Workstation

	xxx		xxx

	~~‘’‘Microscopy~~ – ~~Marleen’’’~~		Microscopy – Marleen

	~~‘’‘Varioscan~~ – ~~Erik’’’~~		Varioscan – Erik

	~~‘’‘Casy~~ Cell ~~Counter’’’~~		Casy Cell Counter

	~~‘’‘TFA’’’~~		TFA

	== Accidents ==		== Accidents ==
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	Current safety regulations must be followed when handling hazardous substances. Specific precautions include:		Current safety regulations must be followed when handling hazardous substances. Specific precautions include:

	~~‘‘Paraformaldehyde’’~~: Must be weighed under the fume hood while in solid form.		''Paraformaldehyde'': Must be weighed under the fume hood while in solid form.

	~~‘‘Mercaptoethanol solutions’’~~: Must only be handled under the fume hood.		''Mercaptoethanol solutions'': Must only be handled under the fume hood.

	~~‘‘Flammable~~ and volatile substances (methanol, ethanol)’’: Stocks must be stored in a safety cabinet. Small quantities (daily use) may be kept in labeled containers at the bench.		''Flammable and volatile substances (methanol, ethanol)'': Stocks must be stored in a safety cabinet. Small quantities (daily use) may be kept in labeled containers at the bench.

	~~‘‘Phenol~~ and phenol-containing ~~solutions’’~~: Must only be handled under Fume Hood II. Special waste containers for liquid and solid/flammable phenol waste are also located there.		''Phenol and phenol-containing solutions'': Must only be handled under Fume Hood II. Special waste containers for liquid and solid/flammable phenol waste are also located there.

	Acrylamide: [Consider adding handling instructions]		''Acrylamide'': [Consider adding handling instructions]

	== Waste Disposal ==		== Waste Disposal ==

ESO wikiadmin at 12:30, 3 June 2025

2025-06-03T12:30:40Z

← Older revision		Revision as of 14:30, 3 June 2025
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	This Page is also available in [[Laborsicherheit\|german]] 🇩🇪.		This Page is also available in [[Laborsicherheit\|german]] 🇩🇪.

	== ~~‘’‘Protective Clothing’’’~~ ==		== Protective Clothing ==
	In the laboratories, it is ALWAYS mandatory to wear a closed lab coat and safety goggles during all procedures. Special caution is required when handling liquid polyacrylamide (casting SDS-PAGE gels) and when working with acids or bases. In such cases, gloves must be worn in addition to the lab coat and goggles.		In the laboratories, it is ALWAYS mandatory to wear a closed lab coat and safety goggles during all procedures. Special caution is required when handling liquid polyacrylamide (casting SDS-PAGE gels) and when working with acids or bases. In such cases, gloves must be worn in addition to the lab coat and goggles.

	== ~~‘’‘Gas’’’~~ ==		== Gas ==
	Lab gas is centrally stored in Room ML in a safety cabinet. The gas supply should be turned off in the evening using the key switch. After completing gas-related work at the lab benches, the gas shut-off valve must be closed.		Lab gas is centrally stored in Room ML in a safety cabinet. The gas supply should be turned off in the evening using the key switch. After completing gas-related work at the lab benches, the gas shut-off valve must be closed.

	== ~~‘’‘Sharp Objects’’’~~ ==		== Sharp Objects ==
	• ‘’‘Needles/Razor Blades/Scalpels’’’ Exercise caution when working with these tools due to injury risk. Do NOT re-cap used needles—dispose of them immediately. These items must be discarded in clearly labeled yellow plastic containers (single-use). One container is located in the cold room, another under Fume Hood II.		• ‘’‘Needles/Razor Blades/Scalpels’’’ Exercise caution when working with these tools due to injury risk. Do NOT re-cap used needles—dispose of them immediately. These items must be discarded in clearly labeled yellow plastic containers (single-use). One container is located in the cold room, another under Fume Hood II.
	• ‘’‘Pasteur Pipettes’’’ Pasteur pipettes should be collected in large, yellow plastic containers (reusable), labeled “Nur Pasteurpipetten / Pasteur Pipettes ONLY”. One container is located in each cell culture lab and one in the cold room. Please DO NOT dispose of other sharp items (scalpels, razor blades, needles, etc.) in these containers, as the contents will be autoclaved and then treated as glass waste.		• ‘’‘Pasteur Pipettes’’’ Pasteur pipettes should be collected in large, yellow plastic containers (reusable), labeled “Nur Pasteurpipetten / Pasteur Pipettes ONLY”. One container is located in each cell culture lab and one in the cold room. Please DO NOT dispose of other sharp items (scalpels, razor blades, needles, etc.) in these containers, as the contents will be autoclaved and then treated as glass waste.
	• ‘’‘Glass Bottles/Broken Glass’’’ Broken glass and empty lab glass bottles should be disposed of in the designated containers.		• ‘’‘Glass Bottles/Broken Glass’’’ Broken glass and empty lab glass bottles should be disposed of in the designated containers.

	== ~~‘’‘Training~~ by Lab ~~Supervisors’’’~~ ==		== Training by Lab Supervisors ==
	‘’‘Working in the Cell Culture Lab’’’		‘’‘Working in the Cell Culture Lab’’’

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	‘’‘TFA’’’		‘’‘TFA’’’

	== ~~‘’‘Accidents’’’~~ ==		== Accidents ==
	On your first visit to the lab, familiarize yourself with the emergency equipment (emergency showers, eye wash stations, fire extinguishers, safety switches), emergency numbers (on the phone next to the elevator), first aid kit (also next to the elevator), and escape routes.		On your first visit to the lab, familiarize yourself with the emergency equipment (emergency showers, eye wash stations, fire extinguishers, safety switches), emergency numbers (on the phone next to the elevator), first aid kit (also next to the elevator), and escape routes.

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	Fire: Memorize escape routes and the designated assembly point.		Fire: Memorize escape routes and the designated assembly point.

	== ~~‘’‘Centrifuges’’’~~ ==		== Centrifuges ==
	Centrifuges may only be operated after instruction by the responsible person (Susanne Feindler-Boeckh). Always balance centrifuge tubes—pre-filled balancing tubes are provided with many centrifuges. Not all tubes are compatible with all rotors. Ensure proper fitting and NEVER force containers into the rotor. Ultracentrifuge rotors must always be operated with ALL buckets in place and properly positioned. Special safety instructions apply when centrifuging suspensions of human-pathogenic bacteria.		Centrifuges may only be operated after instruction by the responsible person (Susanne Feindler-Boeckh). Always balance centrifuge tubes—pre-filled balancing tubes are provided with many centrifuges. Not all tubes are compatible with all rotors. Ensure proper fitting and NEVER force containers into the rotor. Ultracentrifuge rotors must always be operated with ALL buckets in place and properly positioned. Special safety instructions apply when centrifuging suspensions of human-pathogenic bacteria.

	== ~~‘’‘Ethidium Bromide’’’~~ ==		== Ethidium Bromide ==
	DNA staining of agarose gels must be done exclusively in the darkroom using the designated staining/destaining baths. Nitrile gloves are provided there for this specific purpose and MUST NOT be used outside the darkroom. One gloved hand is sufficient, allowing the ungloved hand to operate clean objects (e.g., main power switch).		DNA staining of agarose gels must be done exclusively in the darkroom using the designated staining/destaining baths. Nitrile gloves are provided there for this specific purpose and MUST NOT be used outside the darkroom. One gloved hand is sufficient, allowing the ungloved hand to operate clean objects (e.g., main power switch).

	The water in the destaining bath must be replaced regularly (at least daily). The used solution can be poured into the sink and replaced with deionized water (DI water).		The water in the destaining bath must be replaced regularly (at least daily). The used solution can be poured into the sink and replaced with deionized water (DI water).

	== ~~‘’‘Hazardous Substances’’’~~ ==		== Hazardous Substances ==
	Current safety regulations must be followed when handling hazardous substances. Specific precautions include:		Current safety regulations must be followed when handling hazardous substances. Specific precautions include:

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	Acrylamide: [Consider adding handling instructions]		Acrylamide: [Consider adding handling instructions]

	== ~~‘’‘Waste Disposal’’’~~ ==		== Waste Disposal ==
	Waste from toxic chemicals, dyes, and fixatives must be collected separately in labeled containers (canisters and bottles) before being properly disposed of via the chemical waste facility.		Waste from toxic chemicals, dyes, and fixatives must be collected separately in labeled containers (canisters and bottles) before being properly disposed of via the chemical waste facility.

	== ~~‘’‘Occupational~~ Health ~~Examinations’’’~~ ==		== Occupational Health Examinations ==
	Each employee must undergo a medical examination by the company physician once per year. An initial appointment is arranged through Petra Schnurr when employment begins. All subsequent annual appointments must be scheduled independently. A copy of the fitness certificate must be submitted to the secretariat (Petra Schnurr) after the examination.		Each employee must undergo a medical examination by the company physician once per year. An initial appointment is arranged through Petra Schnurr when employment begins. All subsequent annual appointments must be scheduled independently. A copy of the fitness certificate must be submitted to the secretariat (Petra Schnurr) after the examination.

	== ~~‘’‘Safety Instructions’’’~~ ==		== Safety Instructions ==
	General safety training on biological safety is conducted once a year by Stefan Kreft, the university’s biological safety officer. Additional workplace-specific safety instruction is held once a year in connection with the VTK. Both are mandatory. A safety briefing and lab orientation must also be completed at the start of employment.		General safety training on biological safety is conducted once a year by Stefan Kreft, the university’s biological safety officer. Additional workplace-specific safety instruction is held once a year in connection with the VTK. Both are mandatory. A safety briefing and lab orientation must also be completed at the start of employment.

ESO wikiadmin at 12:29, 3 June 2025

2025-06-03T12:29:55Z

Show changes

ESO wikiadmin at 12:26, 3 June 2025

2025-06-03T12:26:42Z

← Older revision		Revision as of 14:26, 3 June 2025
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	This Page is also available in [[Laborsicherheit\|german]].		This Page is also available in [[Laborsicherheit\|german]] 🇩🇪.

	== General Laboratory Safety Guidelines ==		== General Laboratory Safety Guidelines ==

ESO wikiadmin at 11:57, 3 June 2025

2025-06-03T11:57:38Z

← Older revision		Revision as of 13:57, 3 June 2025
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			This Page is also available in [[Laborsicherheit\|german]].

	== General Laboratory Safety Guidelines ==		== General Laboratory Safety Guidelines ==

ESO wikiadmin at 13:51, 27 February 2025

2025-02-27T13:51:21Z

← Older revision		Revision as of 15:51, 27 February 2025
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	== ~~'''~~General Laboratory Safety Guidelines~~'''~~ ==		== General Laboratory Safety Guidelines ==

	=== ~~'''~~Protective Clothing~~'''~~ ===		=== Protective Clothing ===
	In the laboratory, a fully closed lab coat and safety goggles must ALWAYS be worn when performing any tasks. Special caution is required when working with liquid polyacrylamide (e.g., pouring SDS-PAGE gels), acids/bases, and handling suspended human-pathogenic bacteria.		In the laboratory, a fully closed lab coat and safety goggles must ALWAYS be worn when performing any tasks. Special caution is required when working with liquid polyacrylamide (e.g., pouring SDS-PAGE gels), acids/bases, and handling suspended human-pathogenic bacteria.

	=== ~~'''~~Gas Supply~~'''~~ ===		=== Gas Supply ===
	Laboratory gas is centrally stored in a safety cabinet in room ML639. The supply cylinders should be closed at the end of the day. To open the system, turn the valve half a rotation and then press the individual user button. The gas supply at each workstation is controlled by additional shut-off valves, which must be closed after completing work with gas, as well as the shut-off valve on the Bunsen burner.		Laboratory gas is centrally stored in a safety cabinet in room ML639. The supply cylinders should be closed at the end of the day. To open the system, turn the valve half a rotation and then press the individual user button. The gas supply at each workstation is controlled by additional shut-off valves, which must be closed after completing work with gas, as well as the shut-off valve on the Bunsen burner.

	=== ~~'''~~Sharp Objects~~'''~~ ===		=== Sharp Objects ===

	* '''Needles/Razor Blades/Scalpels:''' Exercise caution when using these tools due to the risk of injury. Do not reinsert used needles into their plastic sheath; instead, dispose of them immediately. These items must be collected in the appropriately labeled yellow plastic containers (single-use containers).		* '''Needles/Razor Blades/Scalpels:''' Exercise caution when using these tools due to the risk of injury. Do not reinsert used needles into their plastic sheath; instead, dispose of them immediately. These items must be collected in the appropriately labeled yellow plastic containers (single-use containers).
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	* '''Glass Bottles/Glass Breakage:''' Broken glass and empty glass bottles should be collected in the designated bins located near the back of room ML639.		* '''Glass Bottles/Glass Breakage:''' Broken glass and empty glass bottles should be collected in the designated bins located near the back of room ML639.

	=== ~~'''~~Centrifuges~~'''~~ ===		=== Centrifuges ===
	Centrifuges may only be operated after receiving proper training from a supervisor. Always balance centrifuge tubes before use; pre-filled balance tubes are available for most centrifuges. Ultracentrifuge rotors must always be operated with ALL buckets in their designated positions. Special operating instructions apply when centrifuging suspensions of human-pathogenic bacteria.		Centrifuges may only be operated after receiving proper training from a supervisor. Always balance centrifuge tubes before use; pre-filled balance tubes are available for most centrifuges. Ultracentrifuge rotors must always be operated with ALL buckets in their designated positions. Special operating instructions apply when centrifuging suspensions of human-pathogenic bacteria.

	=== ~~'''~~Ethidium Bromide~~'''~~ ===		=== Ethidium Bromide ===
	DNA staining of agarose gels with ethidium bromide or GelRed must be performed exclusively in room ML640 using the designated staining/de-staining bath. Nitrile gloves, intended only for this procedure, are provided in this room and must not be used outside of it. One gloved hand is often sufficient, leaving the ungloved hand free to handle ethidium bromide-free objects (e.g., the socket’s main switch). The water in the de-staining bath must be changed regularly, at least once per day.		DNA staining of agarose gels with ethidium bromide or GelRed must be performed exclusively in room ML640 using the designated staining/de-staining bath. Nitrile gloves, intended only for this procedure, are provided in this room and must not be used outside of it. One gloved hand is often sufficient, leaving the ungloved hand free to handle ethidium bromide-free objects (e.g., the socket’s main switch). The water in the de-staining bath must be changed regularly, at least once per day.

	=== ~~'''~~Hazardous Substances~~'''~~ ===		=== Hazardous Substances ===
	Strict adherence to current safety regulations is required when working with hazardous substances. Special precautions apply to:		Strict adherence to current safety regulations is required when working with hazardous substances. Special precautions apply to:

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	* '''Phenol/Phenol-Containing Solutions:''' Must be handled exclusively under a fume hood, where designated containers for the disposal of liquid and solid/flammable phenol-containing waste are provided.		* '''Phenol/Phenol-Containing Solutions:''' Must be handled exclusively under a fume hood, where designated containers for the disposal of liquid and solid/flammable phenol-containing waste are provided.

	=== ~~'''~~Occupational Medical Examination~~'''~~ ===		=== Occupational Medical Examination ===
	All employees must undergo an occupational medical examination once per year. An appointment for the initial examination is arranged through the administrative office upon hiring or starting work. Subsequent annual examinations must be scheduled independently, and a copy of the medical clearance certificate must be submitted to the administrative office.		All employees must undergo an occupational medical examination once per year. An appointment for the initial examination is arranged through the administrative office upon hiring or starting work. Subsequent annual examinations must be scheduled independently, and a copy of the medical clearance certificate must be submitted to the administrative office.

	=== ~~'''~~Safety Training~~'''~~ ===		=== Safety Training ===
	An annual general safety training session on biological safety is conducted by CRH. Additionally, workplace-specific safety training is held once a year in conjunction with the VTK. Attendance at both sessions is mandatory. New employees must also complete safety training and a laboratory orientation upon starting work.		An annual general safety training session on biological safety is conducted by CRH. Additionally, workplace-specific safety training is held once a year in conjunction with the VTK. Attendance at both sessions is mandatory. New employees must also complete safety training and a laboratory orientation upon starting work.

ESO wikiadmin at 22:46, 14 February 2025

2025-02-14T22:46:58Z

@@ Line 1: / Line 1: @@
-= Safety Guidelines =
+== '''General Laboratory Safety Guidelines''' ==
-* Laborsicherheit Allgemein
+=== '''Protective Clothing''' ===
-* GenTZV
+In the laboratory, a fully closed lab coat and safety goggles must ALWAYS be worn when performing any tasks. Special caution is required when working with liquid polyacrylamide (e.g., pouring SDS-PAGE gels), acids/bases, and handling suspended human-pathogenic bacteria.
-* Verhalten im Notfall