View source for Concentration of lentiviral particles via ultracentrifugation

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!Materials
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|Virus containing supernatant
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|20% sucrose in PBS
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|1% BSA in PBS
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=== Procedure ===
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# 4 ml of 20% sucrose solution are added to an ultracentrifuge tube and overlayed with 26 ml of filtrated virus containing supernatant
# the tubes are placed in aerosol tight swing buckets
# centrifugation is carried at 20.000 rpm , 4°C for 2h15min (Rotor: SW32Ti)
# after discarding the supernatant the pellet is resuspended in 250µl 1%BSA
# Aliquot and freeze in liquid nitrogen
# Store at -80°C (repeated freezing and thawing might result in titer reduction)